Klebsiella serotyping by counter-current immunoelectrophoresis
نویسندگان
چکیده
منابع مشابه
Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.
Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum. A result can be obtained within 45 minutes. The optimum conditions for performing the test are given. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type 14. Better precipitin lines are obtained when the gel-support is acid (p...
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Ten serogroups of Legionella pneumophila have now been described.' Although the direct fluorescent antibody technique is widely used to identify and serologically differentiate L pneumophila isolates,2 some cross reactivity between serogroups3 and some staining of non-legionella organisms with legionella antisera4 have been reporrea. Several simple alternatives to the direct fluorescent antibod...
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The counter-immunoelectrophoresis (CIE) method of grouping streptococci was more sensitive than the capillary precipitin method. The precipitate was easier to read, and the test was simple to perform and required fewer reagents. An autoclave-Pronase-B extraction procedure is described and was found superior to either acid, Pronase-B, or the autoclave extraction procedures for recovering groupab...
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Rocket-line immunoelectrophoresis (R-LIE) with antigen containing intermediate gel, and co-agglutination utilising protein A-containing staphylococci coated with specific antibodies, were adapted for serotyping the prototypes of group B meningococci. Both were found to have the same specificity as agar gel double diffusion (AGDD) but they were more sensitive and more rapid than AGDD. R-LIE requ...
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In a study of 188 patients and 109 controls, the detection of antibody by counterimmunoelectrophoresis was used as a diagnostic aid in human infections with Bacteroides fragilis. It was found that positive results indicated current infection and negative results were not conclusive. The method used was simple, rapid, and easily performed in a routine laboratory, but further work is needed to en...
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ژورنال
عنوان ژورنال: Journal of Hygiene
سال: 1978
ISSN: 0022-1724
DOI: 10.1017/s0022172400025043